INTERACTIONS BETWEEN THE MUCOSAL IMMUNE SYSTEM AND THE INTESTINAL MICROENVIRONMENT
The intestinal compartment is a complex environment composed by different type of cells (immune cells, epithelial cells, gut microflora) involved in functional crosstalks aimed at maintaining a balance between tolerance and immunity.
Specifically we are interested to:
- decipher the role of conventional and unconventional intestinal T cells in contributing to tissue homeostasis and in participating to inflammatory immune responses;
- understand the functions of intestinal T lymphocytes in the control of epithelial neoplastic transformations;
- manipulate the function of immune cells for therapeutic purposes.
To do so, we take advantage of a translational approach involving in vitro systems, murine models of intestinal inflammation and of colorectal cancer, and patients’-derived surgical specimens.
In our projects we evaluate the phenotypic and functional status of immune cells isolated from human and murine intestinal samples in homestatic or pathologic conditions, i.e. intestinal inflammation and colorectal cancer. Moreover, we investigate how the intestinal microenvironment, including the gut microbiota, positively or negatively affects T cells activation.
- Nizzoli G, Burrello C, Cribiù FM, Ercoli G, Botti F, Trombetta E, Rescigno M, Paroni M, Caprioli F* Facciotti F*,“Pathogenicity of in-vivo generated intestinal Th17 lymphocytes is IFNγ dependent” , Journal of Crohn’s and Colitis, accepted April 2018
- Cribiù FM, Burrello C, Ercoli G, Garavaglia F, Nizzoli G, Caprioli F, Rescigno M, Bosari S, Facciotti F. “Implementation of an automated inclusion system for the histological analysis of murine models of intestinal inflammation: a feasibility study”. European Journal of Inflammation, accepted April 2018
- Burrello C, Garavaglia F, Cribiù FM, Ercoli G, Bosari S, Caprioli F, Facciotti F “Short-term oral antibiotics treatment promotes inflammatory activation of colonic invariant natural Killer and Conventional CD4+ T cells” Frontiers in medicine Published on 07 February 2018; doi: 10.3389/fmed.2018.00021
- Alfen JS*, Larghi P*, Facciotti F*, Gagliani N*, Paroni M, Maglie S, Gruarin P, Roberto Bosotti R, Iseppon A, Moro M, Crosti MC, Gatti S, Pagani M, Caprioli F, Abrignani S, Flavell R and Geginat J “Intestinal, IFN-γ-producing Tr1-cells co-express CCR5 and PD-1, and down-regulate IL-10 in the inflamed gut of IBD patients” J Allergy Clin Immunol. 2018 Jan 21. pii: S0091-6749(18)30078-2. doi: 10.1016/j.jaci.2017.12.984.
- Biancheri P*., Ammoscato F*., Di Sabatino A., Facciotti F., Caprioli F., Joe-Njoku I., Giuffrida P., Rovedatti L., Corazza GR., MacDonald TT “The role of IL-13 in inflammatory bowel disease”, EJI 2014 Feb;44(2):370-85
- Geginat J, Paroni M, Facciotti F, Gruarin P, Kastirr I, Caprioli F, Pagani M, Abrignani S.“The CD4 centered universe of human T cell subsets.” Semin Immunol. 2013 Nov
- Caprioli F., Marafini I., Facciotti F., Pallone F., Monteleone G. “Targeting T cells in chronic inflammatory bowel diseases” Journal of Clinical & Cellular Immunology, Review, June 2013
- Facciotti F, Ramanjaneyulu GS, Sansano S, Lepore M, Chan RB, Seedorf U, Wenk M, Forss-Petter S, Berger J, Xia C, Mori L, De Libero G “Peroxisome-derived lipids are self-antigens for invariant natural killer T cells” Nature Immunology, Mar 18;13(5):474-8, 2012