Organizer: M. Mapelli (IEO) & A. Casanal (HT) - Dates: 24-28 November 2025
Format: in-person only course
Location: Human Technopole, Milan
The course is meant to illustrate the basic principles of various techniques used in fore-front integrative structural biology approaches, including cryo-electron microscopy and tomography, crystallography, NMR, Mass-Spec Cross-Linking and Alpha-Fold. The topics will be introduced by lectures given by SEMM faculty members and external speakers, complemented with several hands-on tutorials in which the students will familiarize with software and instrumentation.
Crystallization theory and practical
Sebastiano Pasqualato (HT) & Luigi Scietti (IEO)
Basis of crystallography
Marina Mapelli,
IEO
Pymol tutorial
Sebastiano Pasqualato (HT) & Luigi Scietti (IEO)
Structure based drug design
Claudio Ciferri,
Genentech, San Francisco, US
Principles of Cryo-EM SPA
Francesca Coscia,
Human Technopole
Anatomy of the microscopes and sample preparation
Paolo Swuec,
Human Technopole
Challenges of a SPA project
Claudio Alfieri,
ICR, London, UK
Working with Cryo-EM microscopes
Paolo Swuec
Human Technopole
Visit to microscopes
Negative staining tutorial
Students will be divided into 2 groups which will alternate for the microscopes’ visit and negative staining tutorial
Model building tutorial: Coot for model building and structure interpretation of
cryoEM maps
Principles of NMR
Giovanna Musco,
San Raffaele Institute
Mass Spectrometry for Structural Biology: XL-MS
Andrea Graziadei,
Human Technopole
Principles of Alpha-fold
Andrea Graziadei,
Human Technopole
Alpha-fold tutorial
Michelangelo Lassandro and Alessio Di Ianni,
Human Technopole
Principles of Cryo-ET: sample prep and theory
Philipp Erdmann,
Human Technopole
CLEM: correlative light and electron microscopy
Pedro Pereira Machado and Elisa Chen,
Human Technopole
Solist: Biopsie at the nanoscale
Gaia Perone,
Human Technopole
A cryo-ET and cell biology story
Giulia Zanetti,
Crick, London, UK
Cryo-ET workshop and tutorial
Philipp Erdmann,
Human Technopole
Visual Communication in Biology
Margot Riggi,
MPI Martinsried, Germany
Blender
Davide Rambaldi,
Human Technopole
Time for students to work together
Students’ presentation
Ana Casañal (HT) & Marina Mapelli (IEO)