Light Microscopy: theory and techniques | SEMM European School of Molecular Medicine

7-9 April 2025

Mon, 07/04/2025

Milan

Organizers: Simona Rodighiero (IEO) & Dario Parazzoli (IFOM) - Dates: 7-9 April 2025

Location: IEO Campus, Via Adamello 16, Milan

Aim of the course: The course offers a comprehensive introduction to optical microscopy, focusing on fundamental principles, sample preparation, and advanced techniques. Designed for cancer research, it provides participants with the theoretical foundation to visualize cellular and subcellular structures, analyze tumor microenvironments, and apply imaging methods to study cancer biology

Monday, 07 April 2025

Timetable

9.00-12.30

Lesson Argument

Course introduction (Dario Parazzoli, IFOM)
Sample preparation (Sara Barozzi, IFOM)
Fundamentals of optics and wide-field microscopy (Mario Faretta, IEO)

Lesson Location

IEO Silver Room

Timetable

13.30-17.00

Lesson Argument

Tissue imaging: histology (Claudio Tripodo, IFOM)
Optical sectioning – part 1 (Simona Rodighiero, IEO)
Optical sectioning – part 2 (Dario Parazzoli, IFOM)
Wrap-up discussion

Lesson Location

IEO Silver Room

Tuesday, 08 April 2025

Timetable

9.00-12.30

Lesson Argument

Big samples: sample preparation (Fabrizio Orsenigo, IFOM)
Big samples: imaging (Zeno Lavagnino, IFOM)

Lesson Location

IEO Silver Room

Timetable

13.30-17.00

Lesson Argument

Live cell-imaging – part 1 (Sara Barozzi, IFOM)
Lice cell-imaging – part 2 (Ambra Dondi, IEO)
F-techniques (Zeno Lavagnino, IFOM)

Lesson Location

IEO Silver Room

Wednesday, 09 April 2025

Timetable

9.00-12.30

Lesson Argument

Introduction to electron microscopy (Simona Rodighiero, IEO)
Correlative microscopy (Galina Beznusenko, IFOM)
Introduction to super-resolution & cutting-edge microscopy (Nils Gauthier, IFOM)

Lesson Location

IEO Silver Room

Timetable

13.30-17.00

Lesson Argument

“Scientific group” seminar (Andrea Palamidessi, IFOM)
Q&A

Lesson Location

IEO Silver Room