Flow Cytometry: From Introduction to Advanced Applications

23-24 June 2025
Milan

Organizer: Maria Grazia Totaro (IFOM) - Dates: 23-24 June 2025

Location: IEO, Via Adamello 16, Milan

Aim of the course: The aim of course is to provide participants with a comprehensive understanding of the principles, techniques, and applications of flow cytometry. This course is designed for new PhD students who want to learn how to use this technology. By the end of the course, participants should be able to confidently design, execute, and analyse flow cytometry experiments.

Timetable
9.00-13.00
Lesson Argument
  • Fluorescence, fluorochromes and fluorescent dyes (Michela Cravedi, IFOM)
  • How antibodies work (Michela Cravedi, IFOM)
  • Sample preparation for flow cytometry experiments (Maria Grazia Totaro, IFOM)
Lesson Location
IEO Silver Room
Timetable
14.30-16.30
Lesson Argument
  • Introduction to flow cytometry - Conventional flow cytometry (Simona Ronzoni, IEO)
  • A brief introduction to fluorescent activated cell sorting (Simona Ronzoni, IEO)
Lesson Location
IEO Silver Room
Timetable
9.00-13.00
Lesson Argument
  • Spectral flow cytometry (Sara Martone, IFOM)
  • Panel design considerations (Maria Grazia Totaro, IFOM)
  • Data analysis and data presentation (Francesca Clemente, S. Raffaele Institute)
Lesson Location
IEO Silver Room
Timetable
14.30-16.00
Lesson Argument
  • Troubleshooting and round table
Lesson Location
IEO Silver Room

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